Fig. 3
UPLC-MS-ELSD-PDA analysis of fraction 80679-c3 of Berberis thunbergii. a ELSD chromatogram; b PDA chromatogram; c positive ESI–MS TIC chromatogram; d negative ESI–MS TIC chromatogram; e–g positive ESI–MS spectra of the compounds with respective retention times of 0.85, 0.94, and 0.98 min in the PDA chromatogram; h, i negative ESI–MS spectra of the compounds with respective retention times of 0.85 and 0.94 in the PDA chromatogram; j–l UV spectra of the compounds with respective retention times of 0.85, 0.94, and 0.98 min in the PDA chromatogram. UPLC conditions: acquity UPLC BEH C18 column (2.1 × 50 mm, 1.7 μm); gradient elution with 0– 100 % ACN in 0.1 % HCOOH in 3 min