Fig. 4
UPLC-MS-ELSD-PDA analysis of fraction 80679-c5 of Berberis thunbergii. a ELSD chromatogram; b PDA chromatogram; c, d positive ESI–MS BPI and negative ESI–MS TIC chromatograms, respectively; e–g positive ESI–MS spectra of the compounds with respective retention times of 2.06, 2.18, and 2.23 min in the PDA chromatogram; h, i UV spectra of the compounds with respective retention times of 2.18 and 2.23 min in the PDA chromatogram. UPLC conditions: acquity UPLC BEH C18 column (2.1 × 50 mm, 1.7 μm); gradient elution with 0–100 % ACN in 0.1 % HCOOH in 3 min