Fig. 2

Determination of the VAMAX-mix specific titer and quantification of total IgG concentration in serum samples. a The VAMAX-mix-specific titer after immunization of four rabbits in each antigen group (0.1, 1, 10 and 50 µg of VAMAX-mix formulated with Alhydrogel® using a one prime/two boost immunization schedule) were assessed by direct coating ELISA using the VAMAX-mix as the coating antigen. b Total IgG concentration in the pre-immune (P) and immune serum (I) collected on day 70 was quantified by surface plasmon resonance spectroscopy using a protein A capture chip and a quantified rabbit IgG reference for the generation of a standard curve. Gray: 0.1 µg antigen dose; blue: 1 µg antigen dose; green: 10 µg antigen dose; orange: 50 µg antigen dose; square: Rabbit 1 (R1); circle: Rabbit (R2); upward triangle: Rabbit 3 (R3); downward triangle: Rabbit (R4); black horizontal line: geometric mean. For detailed statistical analysis refer to “Methods” section. Asterisks indicate a significant difference (a = 0.05), whereas “NS” indicates no significant differences