Skip to main content

Table 1 PCR primer sequences and reaction conditions were used for the amplification of sequences encoding Plasmodium vivax dhfr and dhps gene

From: Low prevalence of dihydro folate reductase (dhfr) and dihydropteroate synthase (dhps) quadruple and quintuple mutant alleles associated with SP resistance in Plasmodium vivax isolates of West Bengal, India

Gene

Primer name

Primer sequence

Product size (bp)

PCR cycling conditions

Nest I

 pvdhfr

A1F

5′ATGGAGGACCTTTCAGATGTATTTGACATT 3′

720

105 °C for 5 min; (95 °C for 30 s; 50 °C for 30 s; 72 °C for 50 s) × 30 cycles; 72 °C for 10 min

A1R

5′GGCGGCCATCTCCATGGTTATTTTATCGTG 3′

 pvdhps

2A F

5′ATTCCAGAGTATAAGCACAGCACATTTGAG3′

840

104 °C for 5 min; (95 °C for 50 s; 57 °C for 1 min; 72 °C for 1 min) × 35 cycles; 72 °C for 10 min

2A R

5′CTAAGGTTGATGTATCCTTGTGAGCACATC 3′

Nest II

 pvdhfr

A2F

5′TTTATGATGGAACAAGACTGGGACGTT 3′

701

100 °C for 5 min; (95 °C for 45 s; 52 °C for 40 s; 72 °C for 1 min) × 32 cycles; 72 °C for 10 min

A2R

5′TCACACGGGTAGGCGCCGTTGATCCTCGTG 3′

 pvdhps

2 B F

5′AATGGCAAGTGATGGGGCGAGCGTGATT 3′

610

95 °C for 5 min; (94 °C for 40 s; 54 °C for 55 s; 72 °C for 1 min) × 35 cycles; 72 °C for 10 min

2B R

5′CAGTCTGCACTCCCCGATGGCCGCGCCAC 3′