Fig. 2

Image analysis of the clearance of blood from Paracheck Pf RDTs over a 30-min time course after adding 5, 10, 15, 20, or 50 µL of blood directly to the test. a Pixel intensity increases as blood flows onto the lateral flow strip and decreases as the blood is cleared from the strip by the running buffer. b Representative images of RDTs performed with 5, 10, 15, and 20 µL blood after 30 min. Proper RDT development is assessed by visualizing blood clearance from the sample pad and the area around the test and control lines. Time-lapse video of RDT clearance is available as an additional file online