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Table 2 Functional evaluation of antibodies against Baculovirus, Pichia and plant Pfs25 proteins

From: Assessment of Pfs25 expressed from multiple soluble expression platforms for use as transmission-blocking vaccine candidates

Sample

Conca

Mean Oocb

% inhibitionc

p valued

Experiment 1

    

 Baculovirus

750

0.1 (0, 1)

100 (99, 100)

<0.001

 Pichia

750

0 (0, 0)

100 (100, 100)

<0.001

 Plant

750

0 (0, 0)

100 (100, 100)

<0.001

 Plant (reduced)

750

25.1 (3, 45)

3 (−176, 67)

0.945

 Control

750

25.8 (0, 58)

N/A

N/A

Experiment 2

    

 Baculovirus

250

0 (0, 0)

100 (100, 100)

<0.001

 

83

0 (0, 0)

100 (100, 100)

<0.001

 

28

0.4 (0, 2)

98 (94, 100)

<0.001

 Pichia

250

0 (0, 0)

100 (100, 100)

<0.001

 

83

0 (0, 0)

100 (100, 100)

<0.001

 

28

0.3 (0, 2)

99 (95, 100)

<0.001

 Plant

250

0 (0, 0)

100 (100, 100)

<0.001

 

83

0 (0, 0)

100 (100, 100)

<0.001

 

28

0.1 (0, 2)

100 (99, 100)

<0.001

 Control

750

17.1 (0, 62)

N/A

N/A

  1. Groups of ten mice were injected with 10 µg of Pfs25 adjuvanted with montanide on days 0 and 21. On day 42 sera were pooled, and the purified IgGs were tested at the indicated concentrations by SMFA
  2. aIgG concentration (μg/ml) in a feeder
  3. bArithmetic mean (range) of oocysts intensity from 20 mosquitoes
  4. cPercent inhibition of mean oocyst intensity and the 95 % confidence interval (95 % CI)
  5. dTwo-sided p values for testing whether % inhibition is significantly different from 0