Fig. 7

Evaluation of overlapping peptides as B cell epitopes. a ELISA was performed for the nine overlapping peptides with protein-immunized sera at a dilution of 1:1000. Reactivity of recombinant protein and pre-immune sera was used as positive and negative control, respectively. b Kinetics of the sera to peptides 3, 5, 6, and 7 with respect to recombinant protein. c ELISA of PfAARP antisera pre-incubated with different concentrations (25, 50 and 100 µg/ml) of PfAARP, peptide 1, peptide 3, peptide 5 and peptide 7 to PfAARP at a dilution of 1:1000. Reactivity of pre-immune sera and immune sera without pre-incubation was used as controls. White bar indicates the reactivity of day 45 anti-PfAARP sera with PfAARP