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Table 2 Requirement conditions of PfRuvB3 unwinding activity

From: Characterization of Plasmodium falciparum ATP-dependent DNA helicase RuvB3

Factor

Reaction condition

Relative activity (%)

 

Standard reactiona

35

 

−Enzyme

<2

dNTP

−ATP + dATPb

33

 

−ATP + dCTP

<2

 

−ATP + dTTP

<2

 

−ATP + dGTP

<2

Divalent cation

−MgCl2 + CaCl c2

<2

 

−MgCl2 + CuCl2

36

 

−MgCl2 + NiCl2

34

 

−MgCl2 + ZnCl2

30

 

−MgCl2 + FeSO4

<2

 

−MgCl2 + MnCl2

<2

 

−MgCl2 + MgSO4

37

Salt

+NaCl (200 mM)

<2

 

+EDTA (5 mM)

<2

  1. a20 mM Tris–HCl (pH 9.0), 8 mM DTT, 2 mM MgCl2, 2 mM ATP, 10 mM KCl, 4% (w/v) sucrose, 80 mg/ml BSA, 32P-labelled helicase substrate and 1 μg of purified PfRuvB3
  2. b2 mM
  3. c2 mM
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Malaria Journal

ISSN: 1475-2875

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