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Table 1 Primers used for the amplification of PvMCA1-cd, pvmdr1, and Pvcsp of P. vivax isolates

From: Genetic diversity of Plasmodium vivax metacaspase 1 and Plasmodium vivax multi-drug resistance 1 genes of field isolates from Mauritania, Sudan and Oman

Genes

Primers

Sequences

PCR program

PvMCA1-cd

MCA1-F1

5′-CATGGAAACAAAAAAAAGG-3′

95 °C 10′, 35 cycles 94 °C 30″ 56 °C 30″ 72 °C 2′

MCA1-R1

5′-CGAAAACTCCATATCTTTGC-3′

pvmdr1

mdr1-F1

5′-ATAGTCATGCCCCAGGATTG-3′

95 °C 10′, 45 cycles 95 °C 10″, 60 °C 10″ and 72 °C 30″

mdr1-R1

5′-ACGTTTGGTCTGGACAAGTAT-3′

Pvcsp

VCSP-OF

5′-ATGTAGATCTGTCCAAGGCCATAAA-3′

Primary reaction

VCSP-OR

5′-TAATTGAATAATGCTAGGACTAACAATATG-3′

95 °C 10′, 40 cycles 95 °C 1′, 58 °C 2′ and 72 °C 2′

VCSP-NF

5′-GCAGAACCAAAAAATCCACGTGAAAATAAG-3′

Nested reaction

VCSP-NR

5′-CCAACGGTAGCTCTAACTTTATCTAGGTAT-3′

30 cycles 95 °C 1′, 62 °C 2′ and 72 °C 2′

  1. PvMCA1-cd: catalytic domain of Metacaspase1’s P. vivax
  2. Pvmdr1: multidrug-resistant of P. vivax
  3. Pvcsp: circumsporozoite surface protein gene of P. vivax
  4. ’: minute
  5. ”: second
  6. °C: degree Celsius