Genes | Primers | Sequences | PCR program |
---|---|---|---|
PvMCA1-cd | MCA1-F1 | 5′-CATGGAAACAAAAAAAAGG-3′ | 95 °C 10′, 35 cycles 94 °C 30″ 56 °C 30″ 72 °C 2′ |
MCA1-R1 | 5′-CGAAAACTCCATATCTTTGC-3′ | ||
pvmdr1 | mdr1-F1 | 5′-ATAGTCATGCCCCAGGATTG-3′ | 95 °C 10′, 45 cycles 95 °C 10″, 60 °C 10″ and 72 °C 30″ |
mdr1-R1 | 5′-ACGTTTGGTCTGGACAAGTAT-3′ | ||
Pvcsp | VCSP-OF | 5′-ATGTAGATCTGTCCAAGGCCATAAA-3′ | Primary reaction |
VCSP-OR | 5′-TAATTGAATAATGCTAGGACTAACAATATG-3′ | 95 °C 10′, 40 cycles 95 °C 1′, 58 °C 2′ and 72 °C 2′ | |
VCSP-NF | 5′-GCAGAACCAAAAAATCCACGTGAAAATAAG-3′ | Nested reaction | |
VCSP-NR | 5′-CCAACGGTAGCTCTAACTTTATCTAGGTAT-3′ | 30 cycles 95 °C 1′, 62 °C 2′ and 72 °C 2′ |