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Table 1 Groups of mice, vaccine formulations, doses and administration protocols used in Experiments I, II and III

From: Yeast lysates carrying the nucleoprotein from measles virus vaccine as a novel subunit vaccine platform to deliver Plasmodium circumsporozoite antigen

 Group Yeast lysate dose (YU) Adjuvant Number of mice Number of immunizations
Experiment I
 N-PbCS 30 No 8 5
 PbCS 30a No 8 5
 N 30a No 6 5
 WT 30 No 6 5
 Naive / / 8 /
Experiment II
 N-PbCS 30 No 6 3
 N-PbCS 30 No 6 5
 N-PbCS 150 No 6 5
 N-PbCS 300 No 6 5
 N 30a No 6 3
 N 30a No 6 5
 N 150a No 6 5
 N 300a No 6 5
 WT 30 No 4 3
 WT 30 No 4 5
 WT 150 No 4 5
 WT 300 No 4 5
 Naive / / 8 /
Experiment III
 N-PbCS 50 Yes 6 3
 N-PbCS 100 Yes 6 3
 N-PbCS 150 Yes 6 3
 N 50a Yes 6 3
 N 100a Yes 6 3
 N 150a Yes 6 3
 WT 150 Yes 6 3
 Alum / Yes 6 3
 Naive / / 6 /
  1. 1 YU = 107 cells
aDiluted with WT yeast according to the following calculation:
  Expression level, ng/YU Mol. weight, g/mol Expression level, pmol/YU Folds diluted with WT to match N-PbCS Antigen dose in 30 YU, ng
N-PbCS 12 91,32 131 / 360
PbCS 16 33,24 481 3,7 130
N 870 58,08 14,979 114,0 230
  1. 1 YU = 107 cells