Group | Yeast lysate dose (YU) | Adjuvant | Number of mice | Number of immunizations |
---|---|---|---|---|
Experiment I | ||||
N-PbCS | 30 | No | 8 | 5 |
PbCS | 30a | No | 8 | 5 |
N | 30a | No | 6 | 5 |
WT | 30 | No | 6 | 5 |
Naive | / | / | 8 | / |
Experiment II | ||||
N-PbCS | 30 | No | 6 | 3 |
N-PbCS | 30 | No | 6 | 5 |
N-PbCS | 150 | No | 6 | 5 |
N-PbCS | 300 | No | 6 | 5 |
N | 30a | No | 6 | 3 |
N | 30a | No | 6 | 5 |
N | 150a | No | 6 | 5 |
N | 300a | No | 6 | 5 |
WT | 30 | No | 4 | 3 |
WT | 30 | No | 4 | 5 |
WT | 150 | No | 4 | 5 |
WT | 300 | No | 4 | 5 |
Naive | / | / | 8 | / |
Experiment III | ||||
N-PbCS | 50 | Yes | 6 | 3 |
N-PbCS | 100 | Yes | 6 | 3 |
N-PbCS | 150 | Yes | 6 | 3 |
N | 50a | Yes | 6 | 3 |
N | 100a | Yes | 6 | 3 |
N | 150a | Yes | 6 | 3 |
WT | 150 | Yes | 6 | 3 |
Alum | / | Yes | 6 | 3 |
Naive | / | / | 6 | / |
aDiluted with WT yeast according to the following calculation: | |||||
---|---|---|---|---|---|
Expression level, ng/YU | Mol. weight, g/mol | Expression level, pmol/YU | Folds diluted with WT to match N-PbCS | Antigen dose in 30 YU, ng | |
N-PbCS | 12 | 91,32 | 131 | / | 360 |
PbCS | 16 | 33,24 | 481 | 3,7 | 130 |
N | 870 | 58,08 | 14,979 | 114,0 | 230 |