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Table 1 Groups of mice, vaccine formulations, doses and administration protocols used in Experiments I, II and III

From: Yeast lysates carrying the nucleoprotein from measles virus vaccine as a novel subunit vaccine platform to deliver Plasmodium circumsporozoite antigen

 Group

Yeast lysate dose (YU)

Adjuvant

Number of mice

Number of immunizations

Experiment I

 N-PbCS

30

No

8

5

 PbCS

30a

No

8

5

 N

30a

No

6

5

 WT

30

No

6

5

 Naive

/

/

8

/

Experiment II

 N-PbCS

30

No

6

3

 N-PbCS

30

No

6

5

 N-PbCS

150

No

6

5

 N-PbCS

300

No

6

5

 N

30a

No

6

3

 N

30a

No

6

5

 N

150a

No

6

5

 N

300a

No

6

5

 WT

30

No

4

3

 WT

30

No

4

5

 WT

150

No

4

5

 WT

300

No

4

5

 Naive

/

/

8

/

Experiment III

 N-PbCS

50

Yes

6

3

 N-PbCS

100

Yes

6

3

 N-PbCS

150

Yes

6

3

 N

50a

Yes

6

3

 N

100a

Yes

6

3

 N

150a

Yes

6

3

 WT

150

Yes

6

3

 Alum

/

Yes

6

3

 Naive

/

/

6

/

aDiluted with WT yeast according to the following calculation:

 

Expression level, ng/YU

Mol. weight, g/mol

Expression level, pmol/YU

Folds diluted with WT to match N-PbCS

Antigen dose in 30 YU, ng

N-PbCS

12

91,32

131

/

360

PbCS

16

33,24

481

3,7

130

N

870

58,08

14,979

114,0

230

  1. 1 YU = 107 cells