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Fig. 5 | Malaria Journal

Fig. 5

From: Generation of transgenic rodent malaria parasites by transfection of cell culture-derived merozoites

Fig. 5

Success rate of detached cell transfection of Plasmodium berghei parasites. Detached cells and merosomes of wild type or PbmCherryHsp70 parasites were transfected according to the present protocol. a Illustration of the total success rate of detached cell transfection independent of parasites used and constructs transfected. b Success rate of detached cell transfection using DNA constructs allowing integration via either single or c double crossover homologous recombination. d Example of successfully transfected PbmCherryHsp70 parasites with a construct allowing expression of cytoplasmic GFP. Scale bar is 10 µm

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