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Table 2 shows the individual detached cell transfections performed that are summarized in Fig. 5a–c with information about the transfected construct, the parasite line that was transfected, the type of integration, the success rate, how often the transfection was performed and at which day after the transfection the mice became positive

From: Generation of transgenic rodent malaria parasites by transfection of cell culture-derived merozoites

Construct

Parasite line

Integration

Success rate

Number of transfections

Day mice became positive

PbSBP1KO (P0B90-GFP) [19]

mCherryHsp70 [20]

Double crossover

50% (2)

2

7

PbSBP1KO (P0B90-GFP) [19]

mCherryHsp70FLucef1a [21]

Double crossover

100% (1)

2

6

PbSBP1KO (P0B90-GFP) [19]

WT

Double crossover

100% (1)

1

7

PfSBP1 (PL0017-mCherry) [19]

PbSBP1KO [19]

Single crossover

100% (1)

1

8

PbGEM-336027

mCherryHsp70 [20]

Double crossover

100% (1)

1

21

PbNLuc (PL0017) [18]

WT

Single crossover

100% (1)

1

5

PbNLuc (PL0017) [18]

mCherryHsp70 [20]

Single crossover

100% (2)

2

4

PbNLuc (PL0017) [18]

mCherryHsp70FLucef1a [21]

Single crossover

66.6% (3)

3

4–5

PL0017 GFP [22]

mCherryHsp70 [20]

Single crossover

100% (8)

8

5–12