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Table 2 G6PD detection methods, phenotypes and genotypes

From: Safety of primaquine given to people with G6PD deficiency: systematic review of prospective studies

Study (country)

Methods

Results

Phenotype

Genotype

Krudsood [24]

(Thailand)

Not reported

G6PD normal: 134 patients

G6PD deficient: 7 patients

Not tested

Shekalaghe [22]

(Tanzania)

PCR for G202A, A376G

G6PD normal: 77 patients

G6PD deficient: 30 patients

G6PD wild type: 77 patients

G6PD deficient heterozygous: 23 patients

G6PD deficient hemizygous/homozygous: 7 patients

Shekalaghe [23]

(Tanzania)

PCR for G202A, A376G

G6PD normal: 493 patients

G6PD deficient: 69 patients

G6PD wild type: 493 patients

G6PD deficient heterozygous: 47 patients

G6PD deficient hemizygous/homozygous: 22 patients

Rueangweerayut [32]

(Thailand)

PCR RFLP for Mahidol

G6PD normal (Mahidol negative with >80% G6PD activity): 6 patients

G6PD deficient (G6PD enzyme activity 40–60% of normal): 6 patients

G6PD wild type: 6 patients

G6PD deficient heterozygous: 6 patients

Eziefula [27, 33]a

(Uganda)

FST; PCR for G202A/A376G

G6PD normal: 373 patients

G6PD deficient: 88 patients

G6PD wild type: 373 patients

G6PD deficient heterozygous: 61 patients

G6PD deficient hemizygous/homozygous: 27 patients

Kheng [30]

(Cambodia)

FST; enzyme quantification; PCR for Mahidol, Mediterranean, Coimbra, Chinese-5, and Canton

G6PD normal: 57 patients

G6PD deficient: 18 patients

G6PD wild type: 57 patients

G6PD deficient hemizygous male: 14 patients

G6PD deficient homozygous female: 1 patient

G6PD deficient heterozygous female: 3 patients

Bancone [29]

(Thai–Myanmar border)

FST; enzyme quantification; PCR RFLP for Mahidol. Chinese-4, Canton and Viangchan

G6PD normal: 1226 patients

G6PD intermediate: 50 patients

G6PD deficient: 124 patients

G6PD wild type: 1226 patients

G6PD deficient hemizygous male: 39 patients

G6PD deficient homozygous female: 5 patient

G6PD deficient heterozygous female: 65 patients

Ley [31]

(Bangladesh)

FST; enzyme quantification

G6PD normal: 169

Mild G6PD deficiency: 5 patients

Severe G6PD deficiency: 1 patient

Not tested

Mwaiswelo [28]

(Tanzania)

Rapid test; PCR for G202A, A376G

G6PD normal: 184 patients

G6PD deficient: 33 patients

Males

G6PD A wild type: 95 patients

G6PD A− hemizygous: 15 patients

Females

G6PD AA and BA wild type: 83 patients

G6PD A−A− homozygous: 5 patients

G6PD AA− and BA−heterozygous: 22 patients

  1. FST fluorescent spot test, PCR polymerase chain reaction, RFLP restriction fragment length polymorphism (RFLP)
  2. aOnly G6PD ‘normal’ participants based on the FST test; those not normal G6PD by FST were excluded and the genotyping was done on the remainder