Fig. 2

Schematic representation of the sequencing approach by Sanger method to determine the new 18S rRNA gene type sequence of P. ovale spp. Conserved pan-Plasmodium 18S primers (rPLU6 and rPLU5) were first used to amplify the gene spanning three variable regions and two conserved blocks. The nucleotide sequences upstream and downstream of the 454 sequencing target region (V5) of the gene were determined by direct sequencing of the initial product using p-AVL-F and p-AVL-R primers. Partial 18S gene (1078 bp length) was obtained by assembling 454 reads with the sequences obtained using p-AVL-F and p-AVL-R primers