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Table 3 Comparison of nested PCR assay and microscopy results of Plasmodium-infected study participants

From: Absence of Plasmodium inui and Plasmodium cynomolgi, but detection of Plasmodium knowlesi and Plasmodium vivax infections in asymptomatic humans in the Betong division of Sarawak, Malaysian Borneo

Study ID

Gender

Age (years)

Occupation

Malaria history (year of infection)

Temperature

Blood collection period

Microscopy (parasitaemia)

Nested PCR

D001

M

65

Farmer

None

Afebrile

March 2014

32 p/μl

Pk

H011

M

86

Farmer

None

Afebrile

March 2014

48 p/μl

Pk

C028

M

45

Farmer

None

Afebrile

March 2014

48 p/μl

Pk

C024

M

35

Farmer

None

Afebrile

June 2014

Negative

Pk

C031

M

61

Farmer

None

Afebrile

June 2014

48 p/µl

Pk

H030

M

61

Retiree

None

Afebrile

June 2014

Negative

Pv

A008a

M

24

Farmer

None

Febrile (37.4 °C)b

October 2014

3536 p/μl

Pk

A011

F

42

Farmer

Pk (2013)

Afebrile

October 2014

Negative

Pk

H033

F

71

Farmer

None

Afebrile

April 2015

Negative

Genus positive

  1. A, Nanga Mutok; C, Raba Tiput; D, Batu Lintang; H, Nanga Keron; F, female; M, male; p/μl, number of parasites per microlitre of blood; Pk, Plasmodium knowlesi; Pv, Plasmodium vivax
  2. aThis individual had worked at the farm/went hunting the week prior to sample collection
  3. bBody temperature by Thermoflash (Visiomed, France) detection of the temporal artery, which was available from October 2014 onwards