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Table 2 Shows malaria diagnosis for 30 whole blood samples with the illumigene malaria assay in comparison with standard microscopy, RDT and PCR

From: Diagnostic performance of the loop-mediated isothermal amplification (LAMP) based illumigene® malaria assay in a non-endemic region

Sample

Illumigene

RDT

Microscopy

PCR

Parasite density (parasites/µL)

Result

Species

1

Pos

Pos

Pos

Pos

Pf + Po

1,027,798

2

Pos

Pos

Pos

Pos

Pf

292,432

3

Pos

Pos

Pos

Pos

Pv

68,129

4

Pos

Pos

Pos

Pos

Pf

27,731

5

Pos

Pos

Pos

Pos

Pv

7285

6

Pos

Pos

Pos

Pos

Pf

5917

7

Pos

Pos

Pos

Pos

Pf + Po

98

8

Pos

Pos (*)

Pos

Pos

Po

1496

9

Pos

Neg

Pos

Pos

Po

59

10

Pos

Neg

Pos

Pos

Pv

340

11

Pos

Neg

Pos

Pos

Pf

415

12

Pos

Pos

Neg

− (#)

13

Neg

Neg

Neg

/

  

14

Neg

Neg

Neg

/

  

15

Neg

Neg

Neg

/

  

16

Neg

Neg

Neg

/

  

17

Neg

Neg

Neg

/

  

18

Neg

Neg

Neg

/

  

19

Neg

Neg

Neg

/

  

20

Neg

Neg

Neg

/

  

21

Neg

Neg

Neg

/

  

22

Neg

Neg

Neg

/

  

23

Neg

Neg

Neg

/

  

24

Neg

Neg

Neg

/

  

25

Neg

Neg

Neg

/

  

26

Neg

Neg

Neg

/

  

27

Neg

Neg

Neg

/

  

28

Neg

Neg

Neg

/

  

29

Neg

Neg

Neg

/

  

30

Neg

Neg

Neg

/

  
  1. Parasite density as quantified by microscopy on blood smear
  2. RDT, rapid diagnostic test; PCR, four-primer real-time PCR; Pf, Plasmodium falciparum; Pm, Plasmodium malariae; Po, Plasmodium ovale; Pv, Plasmodium vivax; Pos, positive; Neg, negative. –, missing data; (#), negative result with real-time PCR performed on thin blood smear, possibly due to inferior sample quality; (*), difficult to interpret (weak positive result); /, triple negative results (i.e. negative with RDT, microscopy and illumigene malaria assay) were not sent to the ITM for confirmation with real-time PCR