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Fig. 2 | Malaria Journal

Fig. 2

From: Assessment of the quality and quantity of naturally induced antibody responses to EBA175RIII–V in Ghanaian children living in two communities with varying malaria transmission patterns

Fig. 2

Plasmodium falciparum EBA175RIII–V construct and expressed antigen. A schematic representation of the 1620 bp region of EBA175RIII–V cloned into the pLEA2 expression vector, which contains the nucleotide sequence of a hexahistidine tag inframe of the multiple cloning site (a). The culture supernatant (1) containing the secreted protein as well as the purified protein (2) was analysed by SDS-PAGE followed by coomassie staining (b) and a western blot probed with penta-His mouse monoclonal antibody (IgG1) (c)

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Malaria Journal

ISSN: 1475-2875

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