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Table 1 Anopheline species collected from five African countries on which different species-diagnostic PCR analyses were conducted

From: The importance of morphological identification of African anopheline mosquitoes (Diptera: Culicidae) for malaria control programmes

Country

Province/site

GPS coordinates

Morphological ID

Molecular ID using An. gambiae complex PCR

Molecular ID using An. funestus group PCR

Molecular ID using An. funestus-like PCR

Molecular ID using An. rivulorum-like PCR

Botswana

Xakanaka

19°25′32.0″S

23°38′46.0″E

An. wellcomei group (n = 1)

–

–

–

–

Guinea Conakry

Siguiri

11°33′58.1″N

09°25′39.1″W

An. rufipes (n = 8)

–

An. leesoni (5)

–

–

Mali

Yanfolila

11°10′40.8″N

8°09′10.8″W

An. rufipes (n = 10)

–

An. rivulorum (1)

An. leesoni (1)

–

–

Namibia

Katima Mulilo

17°29′48.1″S

24°15′51.5″E

An. squamosus (n = 2)

An. gambiae (2)

–

–

–

South Africa

Kwazulu-Natal

27°26′3.59″S

32°11′25.11″E

An. rufipes (n = 19)

–

–

–

–

 

Gauteng

25°59′23.8″S

27°54′00.2″E

An. crypticus (n = 17)

–

–

–

–

 

An. marshallii

(n = 1)

–

–

–

–

 

Mpumalanga

25°38′15.4″S

31°43′20.6″E

An. coustani (n = 6)

–

–

–

–

 

An. tenebrosus (n = 1)

–

–

–

–

 

An. pretoriensis (n = 20)

–

–

–

–

 

An. rufipes (n = 9)

An. gambiae/An. merus (1)

–

–

–

 

An. maculipalpis (n = 10)

–

–

–

–

 

Limpopo

23°55′ 12.00″S

31°15′ 36.00″E

An. maculipalpis (n = 1)

–

–

–

–

 

An. rhodesiensis (n = 1)

–

An. leesoni (1)

–

–

 

An. listeri (n = 12)

An. gambiae (3)

An. merus (2)

–

–

–

 

Kruger National Park

23°59′18.2″S

31°33′16.9″E

An. pretoriensis (n = 26)

An. arabiensis (1)

An. gambiae (3)

An. funestus (3)

An. vaneedeni (1)

–

–

 

An. rufipes (n = 6)

An. arabiensis (3)

An. gambiae (2)

 

–

–

  1. The total sample size (n) is indicated per morphological species according to each location and the number of specimens that amplified during the PCR analyses is shown for each assay