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Fig. 3 | Malaria Journal

Fig. 3

From: Humanized DRAGA mice immunized with Plasmodium falciparum sporozoites and chloroquine elicit protective pre-erythrocytic immunity

Fig. 3

CPS-CQ immunized DRAGA mice elicit human CD4 and CD8 T cells specific for Pfspz and for iRBCs antigens. Splenic cells and liver mononuclear cells from immunized DRAGA mice were stimulated with Pfspz (a) or with iRBCs (b). Control cultures were unstimulated (nil) or stimulated with un-infected RBC (uRBCs). Cells were stained with human CD3, CD4, CD8, and either IFNγ or TNF Abs and analysed by FACS on the mononuclear FSC/SSC population. Data show frequency of INFγ and TNF secreting cells among gated human CD4 and CD8 T cells. (Blackup-pointing triangle) mice #1&2 (pooled); (Black square) mouse #3, (White square) mice #4&5 (pooled), (Black circle) mouse #6; (White circle) mouse #7. Paired t test p values are shown over the histograms, ns not significant

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