Table 2 Primer sequences and amplification conditions used for nested PCR of pmcsp
Primer name | Sequences (5′ to 3′) | Annealing temperature (°C) | No. of PCR cycle | Product size (bp) | |
|---|---|---|---|---|---|
Nest 1 | Nest 2 | ||||
PmCSF1 | ATGAAGAAGTTATCTGTCTTAGC | 50 | 30 | 1300 | |
PmCSR1 | TTAGTGAAAGAGTATTAAGACT | ||||
PmCSF2 | TTGATTTCCTCTTCCCTGGAT | 52 | 35 | 1250 | |
PmCSR1 | TTAGTGAAAGAGTATTAAGACT | ||||