Fig. 3From: In vitro invasion inhibition assay using antibodies against Plasmodium knowlesi Duffy binding protein alpha and apical membrane antigen protein 1 in human erythrocyte-adapted P. knowlesi A1-H.1 strainGating strategy. a Total cell gating strategy from uninfected erythrocytes with forward scatter-area (FSC-A) and side scatter-area (SSC-A). b Single cell gating from uninfected erythrocytes with forward scatter (FSC-A and FSC-H). c Histogram flow cytometry of uninfected erythrocytes with X-axis FL1-A (SYBR Green I). d Giemsa staining of in vitro culture of P. knowlesi. e Histogram flow cytometry of P. knowlesi invasion. P1, total cells of being counted; P2, single cells of being counted; P3, early rings; P4, late trophozoites and schizonts; P5, infected erythrocytes (P3 + P4)Back to article page