Fig. 4

NO production by BMDM stimulated with HZ after medium replacement. BMDM were primed for 2 h, and treated with HZ (25, 50, or 100 µM), or MDP (2 µg/ml). After 24 h incubation, the supernatants were removed and the levels of nitrite were determined. Fresh medium was added to the cells and further 24 h incubation was performed (24 h + 24 h). Supernatants were collected again, and the nitrite levels determined. Incubation with fresh medium was carried out for further 24 h (24 h + 24 h + 24 h). The supernatants were collected at the end of incubation, and the nitrite levels were determined. ^#P < 0.01 and ^*P < 0.05 vs control medium at each time point; ^**P < 0.01 24 h + 24 h vs 24 h; ^£P < 0.01 24 h + 24 h vs 24 h + 24 h + 24 h. NS not significant. Data represent averages ± SD of three independent experiments