Time (min) | Phase A | Phase B |
---|
0 | 100 | 0 |
5 | 100 | 0 |
8 | 0 | 100 |
20 | 0 | 100 |
21 | 100 | 0 |
28 | 100 | 0 |
- The mobile phases of the LC–MS/MS were water plus formic acid 0.1% (Phase A), and CH3CN plus formic acid 0.1% (Phase B). Gradients, at a flux 0.3 ml/min, were passed through the chromatographic column (Waters Atlantis C185 µm)