Fig. 2

a Overall prevalence of oocysts and sporozoites in midguts (left panel) and salivary glands (right panel) of Anopheles stephensi mosquitoes infected with mature gametocytes of Plasmodium falciparum NF54 from flasks depicted in Fig. 1. Data represents mean ± se. b Prevalence of oocysts and sporozoites from parasites cultured in RBCs collected from four independent donors (indicated by colours) stored for 1-week as refrigerated (“4 °C”, circles) or cryo-preserved (“Cryo”, triangles). NA = not available because sporozoite prevalence for donor 1 was zero due to low corresponding oocyst prevalence at the preceding midgut stages (6%, closed black triangle in b, left panel). Horizontal bars represent group means. See Additional file 3b for prevalence separated by RBC donor and experimental block