Table 1 Outputs of the statistical models
Dependent variable → | Gametocytemia in vitro (male + female stage V) | Oocyst prevalence in mosquito midguts | Sporozoite prevalence in salivary glands | Oocyst abundance (infected and uninfected midguts) | Oocyst intensity (infected midguts only) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Summary statistics → | OR | Std. error | p-value | OR | Std. error | p-value | OR | Std. error | p-value | IRR | Std. error | p-value | IRR | Std. error | p-value |
Fixed effects | |||||||||||||||
(Intercept) | 0.00 | 0.00 | < 0.001 | 1.09 | 0.52 | 0.86 | 0.82 | 0.50 | 0.75 | 1.96 | 0.85 | 0.43 | 5.19 | 0.48 | < 0.001 |
4 °C RBCs vs Cryo-RBCs | 1.31 | 0.41 | 0.40 | 1.05 | 0.38 | 0.89 | 1.35 | 0.84 | 0.63 | 1.20 | 0.71 | 0.80 | 1.12 | 0.36 | 0.75 |
Duration of storage | 1.01 | 0.04 | 0.72 | 0.89 | 0.06 | 0.07 | 0.93 | 0.08 | 0.36 | 0.86 | 0.10 | 0.13 | 0.95 | 0.07 | 0.48 |
Experimental design | |||||||||||||||
Total flasks/mosquito infections (SMFAs), nested within experimental block | 11 | 11 | 10a | 11 | 11 | ||||||||||
Total number of experimental blocks | 5 | 5 | 5 | 5 | 5 | ||||||||||
Total no. of RBC donors tested | 4 | 4 | 4 | 4 | 4 | ||||||||||
Total number of observations | 51 | 283 | 235 | 283 | 157 | ||||||||||
Random effects | |||||||||||||||
Group structure and variance for random effects | |||||||||||||||
Flasks/SMFAs nested within experimental block | 0.00 | 0.00 | 0.26 | 0.43 | 0.10 | ||||||||||
Experimental block | 0.16 | 0.35 | 0.00 | 0.77 | 0.40 | ||||||||||
RBC donor | 0.00 | 0.17 | 0.02 | 0.46 | 0.21 | ||||||||||
Overdispersion tests | |||||||||||||||
Dispersion ratio | 0.98 | 1.23 | 1.00 | 0.75 | 1.07 | ||||||||||
p-value | 0.50 | 0.29 | 0.40 | 1.00 | 0.27 | ||||||||||
Notes | |||||||||||||||
GLMM family (link) | Binomial (logit) | Binomial (logit) | Binomial (logit) | Negative binomial (log) | Negative binomial (log) | ||||||||||