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Fig. 5 | Malaria Journal

Fig. 5

From: Identification of a PH domain-containing protein which is localized to crystalloid bodies of Plasmodium ookinetes

Fig. 5

CryPH is dispensable for ookinete and sporozoite formation and their infectivity. a Erythrocytes (1 × 105) infected with CryPH-cont or ∆CryPH (cl1 and cl2) parasites were inoculated intravenously into 4-week-old female ICR mice pretreated with phenylhydrazine. Parasitemias were examined daily by Giemsa staining. Parasitemia data from five mice are presented as the mean ± standard deviation. b Immunofluorescence assay of PyWT and ΔCryPH using anti-CryPH antibodies (green) and anti-Pys25 antibodies (red). Nuclei were stained by DAPI (blue). Bar: 5 µm. c Immunoelectron microscopy image of ΔCryPH showing normal ookinete apical structure. A crystalloid body (arrow) with a normal structure surrounded by malaria pigments is shown, but gold particles indicating CryPH localization were not observed. C: electron dense collar. Mi: microneme. Bar: 1 µm. d The number of oocysts formed on the mosquito midgut. Mosquitoes (PyWT, n = 20, ΔCryPH, n = 15) were dissected 10 days after they were fed with infected blood. e Sporozoites (1 × 104) collected from salivary glands of CryPH-cont or ∆CryPH (cl1 and cl2) infected mosquitoes were inoculated intravenously into female ICR mice. Parasitemias were examined daily by Giemsa staining. Parasitemia data from five mice are presented as the mean ± standard deviation. No significant difference was observed in the infectivity between CryPH-cont and ∆CryPH sporozoites

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