Fig. 27

Pathway 25 Human health: Potentially cumulative Cas9/gRNA off-target or retargeted nuclease activity in dsxF^CRISPRh transgenics could cause heritable increase in insecticide resistance, fitness or vector competence to increase human disease. The net effect of the population suppression gene drive should ultimately be to reduce this specific harm by reducing the density of mosquitoes including transgenic ones. For this pathway, the first tier of the analysis would involve bioinformatic and molecular assessments of the potential for off-target or retargeted mutations to occur in the transgenic. In the event of such mutations being detected, a second tier of phenotypic characterizations would then be performed. The components of vectorial capacity (V) that would be affected in this pathway are shown in red in the equation