Fig. 5

RBC donor/day effect on Log(GIA₅₀) and level of EoA in GIA₅₀ measurement (a) Indicated numbers of human anti-RH5 pAb (n = 41) were tested at serial dilutions in 3 independent assays using 3 different donors’ RBC, E, F and G. For each sample in each assay, Log(GIA₅₀) was calculated, then the difference (delta) from ave Log(GIA₅₀) in each assay was determined. Each dot indicates the difference for each pAb, and different columns denote data from different assay days (GIA were performed on 5 different days for each donor’s RBC). The same analysis was performed for donors’ H–J (b; n = 17 pAb were tested on 3 different days for each donor’s RBC), K–M (c; n = 30 pAb on 2 different days), N–P (d; n = 34 pAb on 3 different days), and S–U (e; n = 20 pAb on single day). f A ratio between (non-transformed) GIA₅₀ in individual assays and the corresponding (non-transformed) ave GIA₅₀ from three assays (GIA₅₀ Ratio) was calculated for each sample in each assay. The ratio data were divided into 4 groups based on the ave GIA₅₀ level, and each group contains data from 35 or 36 different pAb. Q1, ave GIA₅₀ from 0.7 to 2.1 mg/mL; Q2, 2.2 to 3.6 mg/mL; Q3, 3.6 to 5.4 mg/mL; and Q4, 5.4 to 15.3 mg/mL. The box plot (25/50/75 percentiles) with 2.5/97.5 percentiles (error bars) for each group are shown in a log-scale figure