Laboratory demonstration of a prozone-like effect in HRP2-detecting malaria rapid diagnostic tests: implications for clinical management

Table 2 Detection results of dilutions of cultured parasites on three brands of RDTs in Trial 2

Line & Origin	Parasitaemia %	3% HCT			50% HCT
		ICT	SD	FR	ICT	SD	FR
SJ44	10	4	4	4	4	4	4
Solomon Islands	1	4	4	4	4	4	4
	0.1	4	3	2	3	3	1
	0.01	1	1	0	0	1	0
	0.001	1	0	0	0	0	0
	0.0001	1	0	0	0	0	0
FCR3	16	ND	4	4	ND	4	4
Gambia	1.6	ND	4	4	ND	4	4
	0.16	ND	4	4	ND	4	4
	0.016	ND	4	3	ND	3	2
	0.0016	ND	2	1	ND	1	0
	0.00016	ND	0	0	ND	0	0
GA3	17	4	4	4 thin	4	4	4 thin
Thailand	1.7	4	4	4	4	4	4
	0.17	4	4	4	4	4	4
	0.017	3	3	3	2	3	3
	0.0017	1	1	1	1	1	1
	0.00017	0	0	0	0	0	0
S55	16	ND	4	4	ND	4	4
Solomon Islands	1.6	ND	4	4	ND	4	4
	0.16	ND	4	4	ND	4	4
	0.016	ND	2	2	ND	2	2
	0.0016	ND	1	0	ND	0	0
	0.00016	ND	0	0	ND	0	0
7G8	16	ND	4	4	ND	4	4
Brazil	1.6	ND	4	4	ND	4	4
	0.16	ND	4	4	ND	3	4
	0.016	ND	2	2	ND	2	1
	0.0016	ND	0	0	ND	0	0
	0.00016	ND	0	0	ND	0	0
7G8	25	4 thin	3	4 thin	4 thin	4 thin	4 thin
Brazil	2.5	4	4	4	4	4	4
	0.25	4	4	4	4	4	4
	0.025	4	4	4	4	2	3
	0.0025	1	1	2	2	1	1
	0.00025	1	0	0	0	0	0

Note: ICT- ICT Malaria combo Cassette Test, ICT Diagnostics, South Africa; FR- First Response Malaria Antigen pLDH/HRP2 Combo, Premier Medical Corporation, Limited, India; SD- SD Malaria Antigen P.f, Standard Diagnostics, Korea.

ISSN: 1475-2875