Figure 5

Parasite growth inhibition assay. 3D7 (left column) or Dd2 (right column) sorbitol-synchronized ring-stage-iRBCs were co-incubated with NK92 (A) in different ratios (10:1, 1:1, 3:1 - parasites:cells) in parasite growth medium. Starting parasitaemia was 0,125% in 1% haematocrit. As controls, co-culture was also performed with C32 cells (B), HeLa cells (C), and Jurkat cells (D) in the same conditions. After 24 hours to 48 hours of incubation at 37°C in parasite atmosphere, parasite growth inhibition was quantified by measuring HRP2 release by ELISA. *p < 0.002 for paired comparisons (p < 0.005 - Bonferroni).