Figure 4

(A-C): Plasma levels of IL-10 in P. berghei ANKA infected and control CXCL-10-/- and wild type C57BL/6 mice. CXCL-10-/- and wild type C57BL/6 (n = 10) were infected with P. berghei ANKA parasitized blood intra-peritoneally. Control mice were injected with uninfected blood. Infected and uninfected mice were sacrificed at day 2, 4 & 8. Plasma was collected and ELISA was performed to determine IL-10 levels using the R&D systems (MN USA). IL-10 in plasma of infected mice was higher than in uninfected mice except on day 2 in infected WT (Figures 4A & 4B). Plasma level of IL-10 in infected CXCL-10-/- is significantly up-regulated compared with infected WT at day 2 and 4 post-infection (Figure 4C). Interestingly, IL-10 at day 8 pi in WT plasma was significantly higher in WT than in CXCL-10-/-.