Figure 4From: Simple flow cytometric detection of haemozoin containing leukocytes and erythrocytes for research on diagnosis, immunology and drug sensitivity testingDifferent expression of CD16 on Hz-containing monocytes. PBMCs were isolated from blood of a healthy human volunteer and were incubated with synthetic Hz (0.007 μmol/ml heme-equivalent) and with Plasmodium falciparum Hz (P.f.Hz) at 0.004 μmol/ml heme-equivalent. PBMCs incubated with latex beads and with no Hz were used as controls. CD14+ monocytes were gated, analysed for depolarization and then analysed for CD16 expression. Patient with P. falciparum malaria (0.2% parasitaemia) 2 days into treatment (bottom right panel). Blue shaded area: non-depolarizing monocytes, red line depolarizing monocytes.Back to article page