Figure 9From: Intracellular proteolysis of kininogen by malaria parasites promotes release of active kininsIntracellular calcium mobilization in HUVECs cells after B1 and B2 receptors activation by kinin peptides. HUVECs were loaded with Fluo-3 AM and incubated with HK (20 μg) (HK); 10 μl of parasite culture supernatant without HK (Pf); 10 μl of parasite culture supernatant after incubation with 1.9 μg/μL of HK for 30 min. (Pf + HK); 10 μl of parasite culture supernatant after incubation with 1.9 μg/μL of HK for 30 min and pre-incubation (10 min.) with 4 μM Des-Arg9Leu8-BK (Pf + HK + B1 antagonist); or 10 μM HOE-140 (Pf + HK + B2 antagonist); 10 μl of parasite culture supernatant after incubation with 1.9 μg/μL of HK for 30 min (Pf + HK) pre-incubated with 5 μM E-64 (Pf + HK + E64). Data were normalized as the ratio between measured fluorescence (F) and maximal fluorescence (FmaxTHG) obtained with addition of 5 μM THG. *Denotes statistical significance with respect to all groups values p < 0.01 (n = 3; 30 cells); **Statistical significance of p < 0.05 between Pf + HK and Pf + HK + B2 antagonist (n = 3; 30 cells) in One-way ANOVA test and Newman-Keuls Multiple Comparison test.Back to article page