Figure 2From: Variation in apoptosis mechanisms employed by malaria parasites: the roles of inducers, dose dependence and parasite stagesThe induction of apoptosis in Plasmodium berghei ookinetes following exposure to chloroquine. Ookinetes were maintained in ookinete culture medium only (control) or treated with 1 mM chloroquine for 2 h (chloroquine treated). Following incubation, ookinetes were examined for the presence of four apoptotic markers: condensed chromatin, caspase-like activity, DNA fragmentation and loss of ΔΨm using the acridine orange, CaspaTag, TUNEL and JC-1 assays respectively. Error bars = SEM for three experimental repeats, n = 50–100 ookinetes per sample. Different letters indicate that data are significantly different.Back to article page