Figure 3

3-D reconstruction of P. falciparum parasites. Z-stack images of each parasite shown in Figure 1 were used for 3-D volume rendering and colocalization analysis. No QN colocalization with LysoTracker Red was observed in either the 3D7 (left) or the Dd2 (right) strain. Because LysoTracker Red is highly specific for acidic organelles, QN appears to be in a separate and non-acidic compartment of the food vacuole. QN is shown in blue, LysoTracker Red in red, and the DNA stain DRAQ5 in green.