Skip to main content

Table 1 Detection characteristics of MMSR assay in comparison to “wet” assay

From: Sample-ready multiplex qPCR assay for detection of malaria

 

MMSR assay

Wet assay

Parasite/μL

CTMean

CTSD

CTMean

CTSD

PLU assay

18.55

0.013

19.78

0.191

78240

FAL assay

20.07

0.031

20.85

0.055

 

RNaseP assay

24.13

0.083

30.09

0.081

 

PLU assay

20.73

0.092

22.17

0.227

15648

FAL assay

22.23

0.054

23.62

0.338

 

RNaseP assay

24.52

0.185

29.57

0.082

 

PLU assay

23.58

0.029

24.89

0.616

3129

FAL assay

24.93

0.001

26.06

0.029

 

RNaseP assay

25.37

0.055

29.43

0.492

 
  1. NIBSC standard DNA was five-fold serially diluted times. The performance of MMSR assay was compared to the “wet” assay. The performance of the MMSR assay was comparable to that of the “wet” assay for the three assay targets tested.