Table 1 Sequences of oligonucleotide primers used to amplify merozoite surface protein (msp)-1 and msp-2 polymorphic regions of Plasmodium falciparum isolates from Gabon.
From: Polymorphism in two merozoite surface proteins of Plasmodium falciparum isolates from Gabon
Gene | Primer | Sequence | Notes |
|---|---|---|---|
msp1-C1 | 5'-AAGCTTTAGAAGATGCAGTATTGAC | Conserved | |
msp1-C2 | 5'-ATTCATTAATTTCTTCATATCCATC | Conserved | |
K1a | 5'-GAAATTACTACAAAAGGTGCAAGTG | K1 family-specific | |
msp-1 | K1b | 5'-AGATGAAGTATTTGAACGAGGTAAAGTG | K1 family-specific |
MAD20a | 5'-AAATGAAGGAACAAGTGGAACAGCTGTTAC | MAD20 family-specific | |
MAD20b | 5'-ATCTGAAGGATTTGTACGTCTTGAATTACC | MAD20 family-specific | |
RO33a | 5'-TAAAGGATGGAGCAAATACTCAAGTTGTTG | RO33 family-specific | |
RO33b | 5'-CATCTGAAGGATTTGCAGCACCTGGAGATC | RO33 family-specific | |
msp2-C1 | 5'-ATGAAGGTAATTAAAACATTGTCTATTATA | Conserved | |
msp2-C2 | 5'-CTTTGTTACCATCGGTACATTCTT | Conserved | |
msp-2 | FC27a | 5'-GCAAATGAAGGTTCTAATACTAATAG | FC27 family-specific |
FC27b | 5'-GCTTTGGGTCCTTCTTCAGTTGATTC | FC27 family-specific | |
3D7a | 5'-AGAAGTATGGCAGAAAGTAAGCCTCCTACT | 3D7 family-specific | |
3D7b | 5'-GATTGTAATTCGGGGGATTCAGTTTGTTCG | 3D7 family-specific |