Figure 2From: Assessment of Plasmodium falciparum resistance to ferroquine (SSR97193) in field isolates and in W2 strain under pressureDetection of parasites by flow cytometry analysis. Parasites were double stained with hydroethidine and thiazole orange as described previously [28]. Fluorescence was expressed in Fluorescence Arbitrary Units (FAU) defined by the equipment. A: red blood cell profile. B: Untreated control culture. Red blood cells containing double stained (viable) parasites were visualized in R1. C: Parasites were cultivated without FQ pressure for 8 days. R1 represents the region where the parasite population is detected. Cells outside R1 (left) were unparasitized red blood cells. 38 parasites were counted in a total red blood cell population of 106. D: Parasites were cultivated without FQ pressure for 36 days. Only 2 parasitized RBC were counted in R1 out of 106 red blood cells.Back to article page