Table 3 A. Sensitivity and specificity of all three molecular techniques and microscopy using molecular consensus as the gold standard; and B. microscopy as the gold standard
A | ||||||
|---|---|---|---|---|---|---|
Molecular consensus as gold standard | P. falciparum | P. vivax | Mixed Infection | |||
| Â | Sensitivity (%) | Specificity (%) | Sensitivity (%) | Specificity (%) | Sensitivity (%) | Specificity (%) |
Multiplex | 100 (40/40+0) | 100 (96/96+0) | 92.7 (51/51+4) | 100 (81/81+0) | 0 (0/0+3) | 100 (133/133+0) |
Nested | 100 (40/40+0) | 99 (95/95+1) | 100 (54/54+0) | 100 (82/82+0) | 100 (3/3+0) | 99.2 (132/132+1) |
Real-time | 100 (40/40+0) | 100 (96/96+0) | 100 (51/51+0) | 100 (85/85+0) | 100 (3/3+0) | 100 (133/133+0) |
Microscopy | 92.5 (37/37+3) | 100 (96/96+0) | 90.7 (49/49+5) | 91.5 (75/75+7) | 66.7 (2/2+1) | 98.5 (131/131+2) |
B | ||||||
Microscopy as gold standard | P. falciparum | P. vivax | Mixed Infection | |||
| Â | Sensitivity (%) | Specificity (%) | Sensitivity (%) | Specificity (%) | Sensitivity (%) | Specificity (%) |
Multiplex | 100 (37/37+0) | 97 (96/96+3) | 83.9 (47/47+9) | 95 (76/76+4) | 0 (0/0+4) | 100.00 (132/132+0) |
Nested | 100 (37/37+0) | 96 (95/95+4) | 87.5 (49/49+7) | 93.8 (75/75+5) | 50 (2/2+2) | 98.5 (130/130+2) |
Real-time | 100 (37/37+0) | 97 (96/96+3) | 87.5 (49/49+7) | 93.8 (75/75+5) | 50 (2/2+2) | 99.2 (131/131+1) |