Figure 2

Pedigree criteria for genetic analysis. Pedigrees were chosen for genome-wide genotyping and linkage analysis based on unbiased criteria of infection prevalence (the percent of mosquitoes with at least one midgut oocyst) and pedigree size. These criteria, previously applied in the West African pedigree study [4], prioritize the full analysis of the potentially most informative pedigrees. Pedigrees with ≥20 mosquitoes were analysed (actual range 25–36) and ≥30% infection prevalence (actual range, 31–64%). Only 6 of 28 pedigrees met this criterion, shown in the upper right quadrant. Pedigrees falling outside these boundaries are unlikely to yield a detectable genetic signal, even if segregating strong alleles for resistance/susceptibility. The horizontal line inside the graph indicates 30% prevalence and the vertical line indicates a pedigree size of 20. The red x indicates the pedigree that gave a significant linkage signal.