Figure 1

Affinity purification of r Pf M18AAP. SDS-polyacrylamide gel (left) and immunoblot (right) showing the purification of rPf M18AAP. Lane M – erythrocyte membrane proteins; W – induced E. coli whole cell extract; S – soluble protein fraction after cell lysis; Ub – protein fraction that did not bind to the HIS-Select™ Magnetic Agarose Beads; 1–5 – consecutive 20 mM imidazole washes; E – 200 mM imidazole elution of rPf M18AAP.