Figure 5

Flow cytometric analysis reveals reduction in parasitaemia for both 3D7 and FVO parasite cultures when MSP-1p42 specific antibodies are present. Panel A, B: Changes in DNA content of RBC infected with either 3D7 parasites (Panel A) or FVO parasites (Panel B) cultured either in the presence of anti-MSP-1p42(3D7) (FMP1) (closed circle) or anti-MSP-1p42(FVO) (FMP003) (open circle) (serum concentration = 20% v/v). Infected RBC were stained with Syto-16 and their DNA content determined by flow cytometry and compared to pRBC cultured in the presence of control sera (DNA content = 1). Panel C, D: representative histograms of 3D7 (Panel C) or FVO (Panel D) schizonts after culture with either control sera (black bold line, white area) or MSP-1p42(FVO) (FMP003) (thin line, grey area) specific antisera. Histogram analysis was used to derive the data shown in Panel A, B. Adjuvant used for all three groups was CFA/IFA. One of three representative experiments is shown.