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Table 2 Genotyping of msp1 and msp2 in laboratory lines in different dilutions and mixtures using CE and gel electrophoresis

From: Optimization and validation of multi-coloured capillary electrophoresis for genotyping of Plasmodium falciparum merozoite surface proteins (msp1 and 2)

  

msp1

msp2

  

K1

MAD20

RO33

FC27

IC

Lines

Densitya

CEb

gelc

CEb

gelc

CEb

gelc

CEb

gelc

CEb

gelc

F32

1000

0

0

192.99

205.50

0

0

0

0

524.09

539.10

 

100

0

0

193.01

207.06

0

0

0

0

524.05

539.12

 

50

0

0

192.94

203.59

0

0

0

0

524.07

539.28

 

10

0

0

193.02

201.20

0

0

0

0

524.25

519.80

 

5

0

0

192.99

205.10

0

0

0

0

524.06

540.91

 

1

0

0

0

0

0

0

0

0

0

0

K1

1000

173.48

177.61

0

0

0

0

372.82

392.41

0

0

 

100

173.43

180.28

0

0

0

0

372.85

378.61

0

0

 

50

173.30

179.85

0

0

0

0

372.87

381.90

0

0

 

10

173.64

182.89

0

0

0

0

372.86

382.01

0

0

 

5

173.56

182.51

0

0

0

0

372.85

395.98

0

0

 

1

0

0

0

0

0

0

0

0

0

0

7G8

1000

0

0

0

0

157.06

165.55

0

0

493.14

488.25

 

100

0

0

0

0

157.35

162.95

0

0

493.13

488.21

 

50

0

0

0

0

157.23

167.94

0

0

493.16

472.87

 

10

0

0

0

0

157.33

160.40

0

0

493.19

515.93

 

5

0

0

0

0

157.19

165.92

0

0

0

508.05

 

1

0

0

0

0

0

0

0

0

0

0

F32/K1

1000/10

173.60

177.06

192.98

197.04

0

0

0

0

523.52

509.46

 

500/500

173.66

174.49

192.96

194.87

0

0

372.99

380.85

524.25

521.31

 

10/1000

173.69

182.86

193.02

192.74

0

0

372.96

379.48

524.19

519.79

K1/7G8

1000/10

173.64

178.01

0

0

157.31

152.59

372.97

364.85

493.51

483.32

 

500/500

173.60

175.39

0

0

157.34

161.56

372.98

363.65

493.43

485.01

 

10/1000

173.61

172.85

0

0

157.26

161.57

0

0

493.38

500.08

F32/7G8

1000/10

0

0

193.08

190.99

157.49

0

0

0

524.20/492.68

521.99/0

 

500/500

0

0

193.08

188.05

157.49

165.59

0

0

524.19/493.47

539.46/0

 

10/1000

0

0

193.07

183.33

157.52

163.02

0

0

524.08/493.41

0/493.20

  1. a parasites/ÎŒl
  2. b Fragment siz (bp) including 7 bp tail + A, determined by GeneMapperÂź soft ware after simplex PCR
  3. c Fragment sizes (bp) determined by Quality One 4.4.1 analysis software