Figure 3

Transcript abundances of var genes. (A) Transcript abundances of each var gene shown as a proportion of the total transcript abundance of var genes in sporozoites of clone NF54. Transcript abundances were measured by quantitative PCR using a set of primers that amplify 59 var genes from clone 3D7/NF54. (B) Transcript abundances of each var gene shown as a proportion of the total transcript abundance of var genes and shown as proportion of var types according to the type described by Lavstsen et al [18]: A, B, C, var1, and var2csa. The total transcript abundance of var genes normalized to the average transcript abundance of endogenous control genes, seryl-tRNA synthetase and fructose-bisphosphate aldolase is shown. For comparison, asexual ring stage- and gametocyte stage V parasites showed total transcript abundances of 55 and 3, respectively [13].