Directly-observed therapy (DOT) for the radical 14-day primaquine treatment of Plasmodium vivax malaria on the Thai-Myanmar border

Table 3 Comparison of P. vivax genotypes in primary attack and reappearance(s) (N = 10)

	Case	Primary attack	First reappearance		Second reappearance
		Parasite count (/µl) pvcs, pvmsp3α gene	Day	Parasite count (/µl) pvcs, pvmsp3α gene	Day	Parasite count (/µl) pvcs, pvmsp3α gene
Same*	1	> 400000 c_VK210, A_h8a7	68	160 c_VK210, A_h8a7	91 (159)	800 c_VK210, A_h8a7
	2	8800 be_VK210, A_h6a7	30	6640 be_VK210, A_h6a7	29 (59)	7440 Missing data
	3	3080 c_VK210, A_h1a2	68	16480 c_VK210, A_h1a2
Different ^‡	4	29360 c_VK247, A_hm^†am^†	89	400 b_VK210, A_h1a15
	5	30640 b_VK210, A_h1a4	90	22880 b_VK210, A_h4a6
	6	1280 d_VK210&247, A_h2a3	52	112 c_VK210, A_h5a8
	7	7440 ab_VK210, AC^§	52	12320 b_VK210, A_h7a2
	8	19360 be_VK210, A_h3a1	81	33440 b_VK210, A_h1a4
Ambiguous	9	16560 a_VK210, AC^§	68	2040 c_VK210, C_h1a3
	10	16000 c_VK210, A_hm^†a2	59	2720 c_VK210, A_h5a2	60 (119)	64 c_VK210, A_h1a2

* For two markers, all of the gel picture patterns in the primary-attack parasite and reappearance parasite were the same.
^† Digestion pattern not classified, because sum of PCR-RFLP fragment sizes exceeded uncut gene size.
^‡ For two markers, at least one band in the gel picture patterns in the primary attack and in the reappearance parasite was different.
^§ Digestion pattern not classified because of multiple-genotype infection classified by PCR result for the pvmsp3-α gene.

ISSN: 1475-2875