Figure 1

Identification of plasma MPs. (A) MPs isolated from plasma were gated (R1) based on their forward (FSC) and side (SSC) scatter distribution as compared to 0.7 - 0.9 μm synthetic Nile Red microparticles (grey dots). (B) Events present in R1 were accessed for their annexin V positive staining using PE-conjugated mAbs. Mouse IgG PE-conjugated isotype control mAbs were used to place gates accurately (R2). (C) FITC immunolabeling for the cell markers CD41a (platelets), CD144 (endothelial cells), CD325a (erythrocytes), CD45 (leukocytes) and CD14 (monocytes) was further assessed within annexin V⁺ gated events (R2) and were compared in plasma samples from healthy donors, P. vivax malaria and ovary carcinoma patients (MPs-induced disease). Boxes represent median and interquartile interval, whiskers represent the 10 and 90 percentiles. Means were compared using the Mann-Whitney two-tailed test. A p value < 0.05 was considered significant.