Figure 2

Maximum likelihood phylogenetic trees of An. sundaicus, An. subpictus , and An. vagus genotype B generated from DNA sequences of the rDNA ITS2 (Panel A) and mtDNA cyt-b (PanelB). In Panel A, the ITS2 tree places An. sundaicus from Timor-Leste (TL417) with the An. sundaicus ITS2 sequence variants I-IV. However, the placement of An. vagus genotype B (TL411) specimens basal to the An. sundaicus clade suggest a more recent evolutionary connection to An. sundaicus as the An. subpictus individuals from Papua New Guinea, Indonesia, Vietnam and Sri Lanka appear paraphyletic suggesting several lineages or cryptic species exist within this morphotaxa. In Panel B the Maximum likelihood analysis of cyt-b sequences places An. sundaicus individuals from Timor-Leste (TL415 and TL 417) within other An. sundaicus species forming a well supported and separate branch with individuals from Malaysia/Borneo. The cyt-b analysis also places An. vagus genotype B (TL411 and TL412) basal to the An. sundaicus clade and paraphyletic to An. subpictus individuals, also indicating closer genetic affinities to An. sundaicus and not An. subpictus. For each of the trees only bootstrap branch support values over 70% are displayed.