Table 1 Nucleotides sequences of the primers and probes used for real time PCR. PPIA: Peptidyl Propyl Isomerase A.
From: Chronic infection during placental malaria is associated with up-regulation of cycloxygenase-2
Human Gene | Accession number | Sequences (a: Probe, b: Primer F, c: primer R) | Amplicon size (bp) |
|---|---|---|---|
COX-1 | NM_000962 | a) Fam-CTG GCC TCA GCA CTC TGG AAT GAC AA-Tamra b) ATG ATG GGC CTG CTG TGG A c) CCA ACA CTC ACC ATG CCA AAC | 150 |
COX-2 | NM_000963 | a) Fam-TGC CCA GCA CTT CAC GCA TCA GTT-Tamra b) GCT CAA ACA TGA TGT TTG CAT TC c) GCT GGC CCT CGC TTA TGA | 79 |
15-LOX | NM_001140 | a) Fam-TCC ACC AGG CTT CTC TCC AGA TGT CC-Tamra b) GGA GAC AGT GAT GGC GAC ACT c) TCT GCC CAG CTG CCA AGT | 74 |
PPIA | NM_021130 | a)Fam-AGC TCA AAG GAG ACG CGG CCC A-Tamra b) GTC AAC CCC ACC GTG TTC TT c) CTG CTG TCT TTG GGA CCT TGT | 73 |
IL-10 | NM_000572 | a)Fam-CAG GCA ACC TGC CTA ACA TGC TTC-Tamra b) TGA GAA CAG CTG CAC CCA CTT c) ATC TCC GAG ATG CCT TCA GC | 70 |