Figure 5

Specific reactivity of antibodies produced by the LCLs versus their derived recombinant humAbs. Using ELISA, the specific reactivity of the antibodies from the supernatant of LCLs (undiluted, three-day culture) as well as the derived recombinant humAbs transiently produced in Nicotiana benthamiana against the first (red bars) or second (green bars) EGF-like domain of MSP10 and multi-EGF containing a series of plasmodial EGF-like domains (blue bars). Represented values reflect the reactivity against the fusion proteins corrected by the reactivity observed with the his-tagged control protein (DsRed). Recombinantly expressed and purified humAb10.1 (H5F6:κ5E8), humAb10.2 (H5F6:κ5F6) and humAb10.3 (H5E8:κ5E8) were applied in concentrations of 150, 1.5 and 3 µg/ml, respectively. A pool of Ghanaian plasma samples at a dilution of 1:200 served as positive control, a pool of naïve plasma samples as negative control. Plain B cell medium was used as negative control for the LCL. The ELISA was performed in technical triplicates; the error bars represent the standard deviations.