Figure 7
IFAs of recombinantly expressed humAbs P. falciparum 3D7A schizonts. Binding of humAb10.1, humAb10.2 and humAb10.3 to schizonts was assessed by IFA. Parasites were co-stained with a mouse-anti-MSP4 antibody (green), Hoechst 33342 nuclear stain (blue) and either of the three recombinant humAb10.1 (a), humAb10.2 (b) and humAb10.3 (c) (red). Human and murine antibodies were detected by fluorescently labelled antibodies goat-anti-human IgG (H + L)-Cy3 (Dianova) and goat-anti-mouse IgG (H + L)-Alexa Fluor® 488 (Life Technologies), respectively. Images were taken at a 630× magnification with a Leica TCS SP8 microscope. The right panel shows the overlay of the three fluorescence images. Scale bar 5 µm